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KMID : 0545120200300030398
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Journal of Microbiology and Biotechnology
2020 Volume.30 No. 3 p.398 ~ p.403
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Biosynthesis of Rhamnosylated Anthraquinones in Escherichia coli
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Nguyen Trang Thi Huyen
Shin Hee-Jeong
Pandey Ramesh Prasad
Jung Hye-Jin
Liou Kwang-Kyoung
Sohng Jae-Kyung
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Abstract
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Rhamnose is a naturally occurring deoxysugar present as a glycogenic component of plant and microbial natural products. A recombinant mutant Escherichia coli strain was developed by overexpressing genes involved in the TDP-l-rhamnose biosynthesis pathway of different bacterial strains and Saccharothrix espanaensis rhamnosyl transferase to conjugate intrinsic cytosolic TDP-l-rhamnose with anthraquinones supplemented exogenously. Among the five anthraquinones (alizarin, emodin, chrysazin, anthrarufin, and quinizarin) tested, quinizarin was biotransformed into a rhamoside derivative with the highest conversion ratio by whole cells of engineered E. coli. The quinizarin glycoside was identified by various chromatographic and spectroscopic analyses. The anti-proliferative property of the newly synthesized rhamnoside, quinizarin-4-O-α-l-rhamnoside, was assayed in various cancer cells.
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KEYWORD
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Rhamnosyltransferase, quinizain, Saccharothrix espanaensis
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